Monday, November 25, 2013

LC Biology - Evolution



LC Biology - Genetics


Excellent Resource for introduction to Mendelian Genetics

Friday, November 22, 2013

LC Biology - Osmosis

Please complete this quizlet on Osmosis

Thursday, November 21, 2013

LC Biology Experiments - Osmosis

Hi folks. Please watch the two clips below to revise our Experiment on Osmosis.
Enjoy
Mr Gilmartin.


15 min Time lapse of Experiment

Tuesday, November 19, 2013

Ag Science - Grasslands

Copy in this mindmap to your notes.

Ag Science - Grasslands


Hi folks. Finish these questions on Grasslands for the next day.


Monday, November 18, 2013

LC Biology - Enzyme Revision

Fill out the following test as part of your revision for the Christmas test. Best of luck.

LC Biology Experiments - Enzyme Immoblisation

As part of your homework please check out the two clips below which cover the 2 parts involved in Enzyme Immobilisation.

Part 1 : Immobilisation of Enzyme (Yeast)



Part 2 : Converting Sucrose to Glucose using and Immobilised Enzyme (Yeast)
*Remember the Substrate in this Experiment is Sucrose and the product is Glucose
** Remember that we use clinistixs to check for Glucose at the end of the second clip.

Thursday, November 14, 2013

Ag Science - Revision for Christmas Test

HI folks. Please completed the quizlet below. Make sure to complete the exercise in all forms (Learn, Scatter, Test etc.). It will be good revision for the upcoming Christmas Exam.
Enjoy
Mr. Gilmartin

Wednesday, November 13, 2013

Ag Science - To Compare the Porosity of a Sandy Soil and Clay Soil

LC Biology Experiments - Isolation of DNA

Please view photos from today's experiment on the isolation of DNA from onion cells. The reasons for each step is given below


Chopping onion:
Breaks cell walls, releasing cytoplasm.
Adding washing-up liquid:
Breaks down cell membranes, releases nuclear material from cell.
Adding salt:
Minimise attractive forces between DNA and protein, causes DNA to clump together.
Heating mixture to 60 °C for exactly fifteen mins:
Break down of DNAses enzymes. After 15 mins DNA broken down.
Cooling mixture:
Slowing down any other enzymes before they destroy DNA.
Blending:
Further destroys cell walls and membranes. Causes release of DNA. Blending > 3s cuts DNA strands.
Adding protease:
Breaks down proteins associated with DNA.
Filtering:
Remove large cellular debris. DNA passes through filter with liquid.
Using cold ethanol:
Forms a layer on top of onion filtrate. Alcohol draws water out of DNA molecule, making it less dense - found between two liquids. DNA insoluble in freezing cold ethanol (soluble in alcohol at room temp)
Testing for DNA:
No method of testing for DNA available for use in school labs